Transfection Reagents
BIOSEARCH TECHNOLOGIES INC (Lucigen) TransforMax EC100D pir-116 Electrocompetent E. coli, 10 RxnsPerform plasmid rescue for transposition mutants created with the EZ-Tn5 Transposome Kits.
These cells are used for rescue cloning of DNA transposed using the EZ-Tn5™
Lonza Walkersville Cell Line Nucleofector Kit C
The Cell Line Nucleofector Kit C is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A2058CCRF-CEMMDA-MB-453MEG-01MG-63NCI-H1299 [H1299]NS0 (ECACC)SK-BR-3T2U266B1U-937The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution C0.5 mL Supplement 230 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Cell Biolabs Inc CytoSelect™ 96-Well Cell Transformation Assay Kit (Cell Recovery Compatible, Colorimetric), 96 assays
The traditional soft agar colony formation assay monitors anchorage-independent cell growth by measuring proliferation in a semisolid culture media in tissue culture dishes after 3-4 weeks, followed by manual counting of colonies. This method is cumbersome and not amenable to high throughput, and the manual counting of colonies is subjective because with varying colony sizes, it’s difficult to determine meaningful results. The CytoSelect™ 96-well Cell Transformation Assay (Cell Recovery Compatible) eliminates subjective manual counting of colonies with a much shorter assay time. Cells are incubated only 6-8 days in a semisolid agar media before being solubilized, lysed and detected by the provided colorimetric dye in a standard plate reader, making it a more quantitative and higher-throughput method. Additionally, the proprietary medium makes it possible to recover viable and intact cells for further downstream analysis. Each kit is sufficient for 96 tests in a microtiter plate.
Sigma Aldrich Fine Chemicals Biosciences REAGENT KIT FOR MRP2 MRP3 MRP5
NC3883695 REAGENT KIT FOR MRP2 MRP3 MRP5
Agilent Technologies GENEJAMMER TRANSFECT REAGENT
GeneJammer Transfection Reagent 1mL 1.33 mg/mL reagent in 80% ethanol Applications Incorporation of foreign genes into mammalian cells including primary cultures and hard-to-transfect cell lines Transfects DNA
Agilent Technologies GENEJAMMER TRANSFECT REAGENT
GeneJammer Transfection Reagent is a proprietary formulation of polyamine and other components in 80% ethanol ideal for transient or stable transfections in a wide variety of cell lines .Fast, easy to use with low cytotoxicity
Agilent Technologies GENEJAMMER TRANSFECT REAGENT
GeneJammer Transfection Reagent Sampler 0.3mL 1.33 mg/mL reagent in 80% ethanol Applications Incorporation of foreign genes into mammalian cells including primary cultures and hard-to-transfect cell lines Transfects DNA
Bio Rad Laboratories STOPPING SCREENS,500/PK
Stopping Screens for use with the PDS-1000/He system.Pkg of 500
Sciencell Research Laboratories RAT EPIDRMAL KERATINOCYTES 1ML
NC3451200 RAT EPIDRMAL KERATINOCYTES 1ML
Kyfora Bio DMG-PEG2000 100 MG
DMG-PEG 2000 (1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000) is a PEGylated lipid used in the formation of lipid nanoparticles (LNP) for the development of mRNA vaccines such as COVID-19 vaccine and for the delivery of nucleic acids such as RNAis. It is derived from dimyristoyl glycerol (DMG) which behaves similar to phospholipids, namely in the way they associate with membranes. PEGylation of the lipid enhances bioavailability and prevents opsonization of the LNP. This product is for research use only. Key application(s): mRNA vaccine, gene delivery
Kyfora Bio DMG-PEG2000 1 GR
DMG-PEG 2000 (1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000) is a PEGylated lipid used in the formation of lipid nanoparticles (LNP) for the development of mRNA vaccines such as COVID-19 vaccine and for the delivery of nucleic acids such as RNAis. It is derived from dimyristoyl glycerol (DMG) which behaves similar to phospholipids, namely in the way they associate with membranes. PEGylation of the lipid enhances bioavailability and prevents opsonization of the LNP. This product is for research use only. Key application(s): mRNA vaccine, gene delivery